CRISPR-Cas9 System

The CRISPR-Cas9 system is a powerful and adaptable tool for gene editing in mammalian cells, enabling precise gene knock-out (KO) and knock-in (KI) applications. Originally derived from the bacterial immune system, it has been optimized for ease of use with all-in-one plasmids that co-express Cas9 and guide RNA (gRNA), and often include EGFP for visual confirmation of transfection. Researchers can use pre-designed Duo-gRNA sets or clone their own sequences for targeted editing. For knock-in studies, homologous recombination is supported via donor plasmids containing GFP-Puromycin cassettes flanked by homology arms. The system is compatible with multiple delivery methods, including transient transfection, lentiviral, and AAV vectors. Cas9 expression vectors with nuclear localization signals ensure effective targeting, and options are available for gene editing in human, mouse, and rat models. Although CRISPR is efficient, expert support is recommended to guide users through design, cloning, and delivery steps for optimal results. - kits